Figure 4 | Cell Death & Disease

Figure 4

From: The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury

Figure 4The alternative text for this image may have been generated using AI.Figure 4The alternative text for this image may have been generated using AI.

Adoptive transfer of MDSCs protects kidney against AKI and mTOR signal inhibition enhances MDSCs’ protective effects. (a) The level of serum creatinine (μmol/l) and blood urea nitrogen (mmol/l) were decreased after adoptive transfer of MDSCs and further decreased after adoptive transfer of rapamycin-treated MDSCs. (b) Renal histologic damage was assessed in H&E-stained sections. Mild-to-moderate tubular vacuolation and hemorrhage were observed in the kidneys in the MDSC group compared with the IR group, and the histologic damage of kidneys in Rapa+MDSC group was even milder. (c) The percentage of apoptotic cells (shown as dark purple in color) in kidney tissues in situ was detected and quantified by using TUNEL assay. (d) Flow cytometry analysis showed the percentage of infiltrated CD4+ T cells in kidney tissues after adoptive transfer of non-rapamycin-treated MDSCs and rapamycin-treated MDSCs. (e) The expression of IL-1β, IL-6, IFN-γ, TGF-β1 and Foxp3 mRNA in the kidney was examined by RT-qPCR. (f) The serum level of cytokine IL-1β, IL-6, IFN-γ and TGF-β1 was measured by using Luminex and ELISA kit. (data were shown as mean±S.D.; n=5 mice per group; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; TUNEL, TdT-mediated dUTP Nick-End Labeling)

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