Figure 2 | Cell Death Discovery

Figure 2

From: Helicobacter pylori VacA induces apoptosis by accumulation of connexin 43 in autophagic vesicles via a Rac1/ERK-dependent pathway

Figure 2

Increase of ubiquitinated Cx43 by VacA. (a) Cells were treated with 10 μM MG132, 10 μM lactacystin for 30 min, followed by incubation with heat-inactivated (iV) or wild-type VacA (V) for 10 h and subjected to immunoblots using the indicated antibodies. A blot representative of three separate experiments is shown. Quantification of VacA-induced Cx43 levels in AZ-521 cells was performed by densitometry (bottom panel). Data are presented as mean±S.D. of values from three experiments and significance is *P<0.05. (b) Cells were treated with 120 nM heat-inactivated (iV) or wild-type VacA (V) for 10 h, lysed with RIPA buffer, immunoprecipitated with anti-Cx43 antibodies and subjected to immunoblotting using anti-ubiquitin FK1 or P4D1 antibodies and anti-Cx43 antibody as described in Materials and Methods. Experiments were repeated two times with similar results.

Back to article page