Figure 2
From: CAML mediates survival of Myc-induced lymphoma cells independent of tail-anchored protein insertion
Inhibition of apoptosis in Caml-deleted cells does not restore proliferation. (a) Growth curves for Eμ-Myc;Cre-ER;Camlfl/fl cell lines treated with vehicle (EtOH) or 4-OHT, and vehicle (DMSO) or caspase inhibitor Q-VD-OPh (QVD) (E2409, 25 μM; ECF, 50 μM). Values are mean±S.E.M. of two independent replicates from representative data that gave similar results. (b) Loss of caspase-3 and PARP cleavage by QVD treatment. Lysates were collected from parallel cultures of a at 48 h, and protein expression was analyzed by western blot. (c) Growth curves indicate proliferation is not rescued by Bcl-2/xL. Values are mean±S.E.M. of two independent replicates from representative data that gave similar results. (d) Western blot for parallel cultures of c showing loss of cleaved caspase-3 and PARP in Bcl-2/xL-overexpressing cells. (e) Annexin V and PI staining of Caml-targeted, Bcl-2/xL-overexpressing cells indicate rescue from cell death. Eμ-Myc;Cre-ER;Camlfl/fl (E2409) Bcl-2/xL-overexpressing cells were treated with EtOH or 4-OHT and stained with annexin V and PI at 56 h. Statistical differences between EtOH and 4-OHT treatments, for time points starting at 72 h, are denoted by *P<0.05, **P<0.01, and ***P<0.001. Lack of significance is not indicated.
