Figure 2
From: Involvement of proapoptotic genes in autophagic cell death induced by irradiation

Apoptosis of Bax−/− and PUMA−/− HCT116 cell after irradiation. Irradiation of 8 Gy was given to WT, Bax−/− and PUMA−/− HCT116 cells, and after 72 h of incubation, apoptosis was determined by Annexin-V and PI staining with flow cytometry. Representative flow cytometric plots (a) and the quantification (b, mean±S.D.) are shown. Con, control, no irradiation. IR, irradiation. Statistical significant differences are marked as *P<0.05 or **P<0.01.