Figure 1
Polarization level of various PM cargos. (a–f) Relative fluorescence intensities showing predominantly a basal localization of PIN1-GFP in stele cells (a), an apical localization of PIN2-GFP in epidermal cells (b), an outer lateral localization of GFP-ABCG37 in epidermal cells (c), an outer lateral localization of ABCG36-GFP in epidermal cells (d), an inner-lateral localization of BOR1-GFP in epidermal cells (e), and an uneven distribution of PIP2-GFP (f). The relative fluorescence intensities are color coded from 0 (black) to 250 (bright/white). Scale bars=10 μm. (g) Quantification of the polarity index for steady-state PIN1-GFP, PIN2-GFP, GFP-ABCG37, ABCG36-GFP, and BOR1-GFP. The polarity index was calculated as the maximal signal ratio between the defining polar domain (full white rectangle) and it’s adjacent or opposite domain (dashed white rectangle). In the case of PIN1-GFP, the signal at the lateral domain was divided by 2 accounting for the equal contribution of fluorescent signal from two adjacent membranes. (h) Quantification of the normalized polarity index for steady-state PIN1-GFP, PIN2-GFP, GFP-ABCG37, ABCG36-GFP, and BOR1-GFP. Normalized index was generated by dividing the initial polarity index of each marker (g) by the corresponding PIP2-GFP polarity index (Supplementary Figure S1B). Error bars represent s.e.m.; P-value calculated according to Student’s t-test. n=80–100 cells from 20 to 25 roots.
