Figure 1

Long-term consumption of caffeine-free HSCBs aggravates the pathogenesis of EAE. (a) Mice were treated with individual cola beverages for 8 weeks and immunized with MOG35-55 for the induction of EAE. Clinical score was assessed daily and shown (n=10). (b–e) 18 days post immunization, lumbosacral spinal cords were isolated and performed H&E (d) or Luxol fast blue (e) staining for assessment of histopathology. Representative sections (d, e) and statistical analysis (b, c) data are shown. Infiltration of lymphocytes and demyelination are highlighted by arrow, and scale bars are present as 250 μm. All data above are representative of three independent experiments. ns, not significant.