Figure 2

HSCBs boost Th17 responses in EAE. Lymphocytes from CNS or inguinal LN were isolated 18 days post immunization and used for assessment of different CD4 T cell subsets. (a) Representative staining of CNS-infiltrated inflammatory CD4 T cell subsets, gated on TCRβ⁺ CD4⁺. (b) Statistical analysis of the percentages of IL-17A⁺ GMCSF⁺ double positive, IL-17A⁺ IFN-γ⁺ double positive, total IL-17A⁺ (both IL-17A⁺ GMCSF⁻ and IL-17A⁺ GMCSF⁺), total GMSCF⁺ (both IL-17A⁻ GMCSF⁺ and IL-17A⁺ GMCSF⁺) and total IFN-γ⁺ (both IL-17A⁻ IFN-γ⁺ and IL-17A⁺ IFN-γ⁺) in a. (c and d) Representative staining of different CD4 T cell subsets in inguinal LN, gated on TCRβ⁺ CD4⁺. (e and f) Statistical analysis of data in c, d. (g) Mice consuming individual beverages were i.p. injected with anti-IL-17A antibodies (n=5) or isotype control antibodies (n=10) in the induction and duration of EAE. The clinical score was assessed daily and shown. All data above are representative of at least two independent experiments.