Figure 3

HSCBs induce a disease-prone structure of microbiota. (a) Mice were treated with individual cola beverages for 8 weeks and feces were used for isolation of 16s rRNA and subsequent sequencing analysis. Unweighted Unifrac principal coordinates analysis plots of each sample and the distance of each group are shown (n=10). Statistical analysis (b) and representative staining (c) of IL-17 in CD4 T cells isolated from different organs of mice without immunization, gated on TCRβ⁺ CD4⁺. (d) Mice were depleted of microbiota after Cola beverages consumption and immunized for EAE disease. Clinical score was assessed and shown (n=7). (e) Microbiota-depleted mice were transplanted with feces from mice consuming individual cola beverages or water and 16s rRNA analysis of the recipient mice was performed. (f) The feces recipient mice in (e) were induced for EAE disease. Clinical score was assessed and shown (n=7). (g and h) Representative staining and statistical analysis of Th17 level in the colon in response to fecal transplantation, gated on TCRβ⁺ CD4⁺. All data above are representative of at least two independent experiments. Abs, antibiotics.