Figure 4

HeLa cells were infected with AdCMV-luci, AdTRE-E1 and AdCMV-E1 without Dox. These cells were also co-infected with AdCMV-rtTA and AdTRE-E1 with or without Dox. At 3 days after infection, 50 μl of the culture medium was removed and subjected to plaque-forming unit assay. HeLa cells co-infected with AdCMV-rtTA and AdTRE-E1 produced significantly larger amounts of progeny viruses (P<0.05) with Dox. Mean±s.e. of triplicate determination is shown.