Figure 1 | Cancer Gene Therapy

Figure 1

From: RETRACTED ARTICLE: VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Figure 1

Cytolysis of HeLa cells requires the presence of the VP2 domain of the H-1 parvovirus during H-1 infection. (a) HeLa and NRK cells were infected with chimeric viruses (CH1–5) as well as the parental KRV and H-1 parvoviruses (multiplicity of infection=1). The cell viability was observed by light microscopy for 72 h after infection. The viable cells were counted with trypan blue exclusion 72 h after infection. Average of results from triplicate wells were obtained, and the error bars indicates s.e.m. (*P<0.01; H-1, CH4 or CH5 vs KRV). (b) For construction of chimeric viruses, common restriction enzymes were used on the H-1 and KRV sequences after sequencing KRV genome. KRV genomic fragments were replaced with those of H-1 virus in the pSR19 vector. NRK cells were transfected with the recombinant vectors and isolated for cytolysis and the chimeric viruses were harvested.

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