Figure 3

Cytolysis of HeLa cells results from a preferential amplification of H-1, CH4 and CH5 virus. (a and b) HeLa and NRK cells were infected with chimeric viruses (CH1–5), and the parental KRV and H-1 parvoviruses (multiplicity of infection (MOI)=1). The cell lysates were prepared 48 and 72 h after infection, and the VP2 capsid proteins of KRV and H-1 viruses were detected using polyclonal H-1 antibodies.