Figure 4

Viral transcription and replication occurs preferentially in HeLa cells infected with H-1, CH4 and CH5 virus. (a) Viral RNA transcripts were isolated from HeLa or NRK cells infected with chimeric viruses (CH1–5), and the parental KRV and H-1 parvoviruses (3 × 10⁶ viral DNA copy per ml) 12 h after infection. Viral transcript was measured by qRT-PCR, using NS1 primer following synthesis of cDNA, and analyzed with CFX96 Real-Time PCR Detection System. The viral transcript was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and the normalized amount of viral transcript from H-1-infected HeLa cells or NRK cells was set to 1 (*P<0.01; H-1, CH4 or CH5 vs KRV). (b) Viral genomic DNA was isolated from HeLa or NRK cells infected with chimeric viruses (CH1–5), and the parental KRV and H-1 parvoviruses (3 × 10⁶ viral DNA copy per ml) 1 and 24 h after infection using a Viral DNA Purification Kit. Viral DNA was measured by qPCR using NS1 primer, and normalized to linear pSR19 plasmid (*P<0.01; H-1, CH4 or CH5 vs KRV).