Figure 3
T-cell effector function upon adoptive transfer. (a) On day 3 after adoptive transfer, the OT-I cells were isolated from the liver and stimulated in vitro with Ova peptide to induce effector cytokine expression. Seven hours after stimulation, the cells were stained for the effector cytokines IFNγ (upper panel) and TNFα (lower panel). The expression levels were compared with those of Ova single transgenic control mice. (b) Quantification of IFNγ/TNFα double positive OT-I cells isolated from the livers and spleens of Ova × CreERT2 mice on day 3. (c) Investigation of cytokine expression in OT-I cells on day 13 following adoptive transfer. Cells from the liver were treated and analyzed as described in (a). IFNγ (upper panel) and TNFα (lower panel) release was investigated. (d) Quantification of the IFNγ/TNFα double positive OT-I cells in the livers and spleens of the respective recipient groups is shown. (e) In vivo cytotoxic activity of OT-I cells in the periphery of adoptively transferred recipient mice. OT-I recipients were challenged with Ova-pulsed (CFSEhi) and control (CFSElo) splenocytes on days 13 (left panel) and 27 (right panel). Eighteen hours after transfer, the CFSE-labeled cell populations in the spleen were analyzed by flow cytometry to investigate the specific lysis percentage. Results from n ≥ 5 mice/group ± SD from two independent experiments are shown.
