Abstract
Changes in the distribution of 1Pl-antigen in the developing chick retina have been examined by indirect immunofluorescence staining technique using the novel monoclonal antibody ( MAb) 1P1. Expression of the 1P1 antigen was found to be regulated in radial as well as in tangential dimension of the retina, being preferentially or exclusively located in the inner and outer plexiform layers of the neural retina depending on the stages of development. With the onset of the formation of the inner plexiform layer 1P1 antigen becomes expressed in the retina. With progressing differentiation of the inner plexiform layer 1P1 immunofluorescence revealed 2 subbands at E9 and 6 subbands at E18. At postnatal stages (after P3) immunoreactivity was reduced in an inside-outside sequence leading to the complete absence of the 1P1 antigen in adulthood. 1P1 antigen expression in the outer plexiform layer was also subject to developmental regulation. The spatio-temporal pattern of 1P1 antigen expression was correlated with the time course of histological differentiation of chick retina, namely the synapse rich plexiform layers. Whether the 1P1 antigen was functionally involved in dendrite extension and synapse formation was discussed.
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References
Cowan WM . Aspects of neuronal development. In: (R.Porter ed.) International Review of Physiology. Neurophysiology III, vol 17, Baltimore: University Park Press, 1978:149–91.
Gordon-Weeks PR . GAP-43-what does it do to the growth cone? TINS 1989; 12:363–5.
Valentino KL, Winter J and Reichardt LF . Applications of monoclonal antibodies to neuroscience research. Annu Rev Neurosci 1985;8:199–232.
Schlosshauer B, Diitting D, Wild M . Target-independent regulation of a novel growth associated protein in the visual system of the chicken. Development 1990; 109:395–409.
Schlosshauer B, Wild M . Generation of monoclonal antibodies specific for developmentally regulated antigens of the chicken retina. Dev Brain Res 1991; 59:197–208.
Schlosshauer B, Dütting D . Intraretinal pathfinding of ganglion cell axons is perturbed by a monoclonal antibody specific for a G4/Ng-CAM-like cell adhesion molecule. Dev Brain Res 1991;63: 181–90.
Fazekas de St, Groth S, Scheidegger D . Production of monoclonal antibodies: strategy and tactics. J Immunol Meth 1980;35:1–21.
Diitting D, Gierer A, Hansmann G . Self-renewal of stem cells and differentiation of nerve cells in the developing chick retina. Dev Brain Res 1983; 10:21–32.
Sanes JR . Extracellular matrix molecules that influence neural development. Ann Rev Neurosci 1989; 12:491–516.
Ehinger B, Dowling JE . Retinal neurocircuitry and transmission. In: (A Bjorklund, T Hokfelt, LW Swanson eds.) Handbook of Chemical Neuroanatomy. Vol. 5:Integrated Systems of the CNS, Part I. New York, Elsevier Science Publishers B. V., 1987:389–447.
Ramon Y, Cajal S . The structure of the retina (Translated by S.A. Thorpe and M. Glickstein). Springfield, III., Charles C. Thomas.1933.
Halfter W, Reckhans W and KrSger S . Nondirected axonal growth on basal lamina from avian embryonic neural retina. J Neurosci 1987; 7::3712–22.
Acknowledgements
This work was supported by the German Max-Planck-Gesellschaft and the National Natural Sciences Foundation of China No.3897035. We thank Li-zhen Jiang, Hui-yi Miao and Hui Zhu for their excellent technical assistance.
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*This work was completed in Max-Planck Guest Laboratory at the Shanghai Institute of Cell Biology, Academia Sinica, as a collaborative project of 3 Institutes.
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Wang, H., Song, Q. & Burkhard, S. Spatiotemporal distribution of 1P1 antigen expression in the plexiform layers of developing chick retina. Cell Res 3, 187–193 (1993). https://doi.org/10.1038/cr.1993.20
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DOI: https://doi.org/10.1038/cr.1993.20
