Abstract
Mature eggs (at metaphase II stage) produce a series of Ca2+ oscillation at fertilization. To define whether the fertilization-induced Ca2+ oscillation is restrict to the metaphase II eggs and cell cycle dependent, mouse oocytes at prophase I (arrested at germinal vesicle stage), metaphase I, metaphase II, as well as the pronuclear embryos at interphase of the first mitotic division derived from fertilization or parthenogenetic activation were inseminated after removal of zona pellucida. The results show that the fertilization-induced Ca2+ oscillation is not specific to metaphase II eggs. This is supported by the fact that immature oocytes generated the Ca2+ oscillations at fertilization regardless of their nuclear progression from prophase I to metaphase I (in vitro matured) stage. More interestingly, it was first found that pronuclear embryos at interphase derived from parthenogenetic activation showed Ca2+ oscillations in response to fertilization while the zygotes at interphase did not after reinsemination or intracytoplasmic injection of sperm extracts which induce Ca2+ oscillations in MII eggs. This suggests that the ability of oocytes to generate Ca2+ oscillation in response to sperm penetration is not regulated in a cell cycle dependent manner but dependent on the cytoplasmic maturation.
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References
Whitaker M, Swann K . Lighting the fuse at fertilization. Development 1993; 117:1–12.
Kline D, Kline JT . Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg. Dev Biol 1992; 149:80–9.
Sun FZ, Hoyland J, Huang X, Mason W, Moor RM . A comparison of intracellular changes in porcine eggs after fertilization and electroactivation. Development 1992; 115:947–56.
Deng MQ, Fan BQ . Intracellular Ca2+ changes during fertilization and artificial activation. Acta Pharmacologica Sinica 1996; 17(4):357–60.
Jones KT, Carroll J, Merriman LA, Whittingham DG, Kono T . Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent. Development 1995; 121:3259–66.
Deng MQ, Fan BQ, Liu YM, Wang JX . Intracellular calcium changes induced by fertilization and artificial activation and their roles in oocyte activation. Acta Zoologica Sinica 1996; 42(1):80–6.
Deng MQ, Xin J, Huang XY, Sun FZ . Sperm-induced Ca2+ oscillations in mouse maturing oocytes and postovulatory aging eggs. Chinese Science Bullet in 1996 (in press).
Hogan B, Costantini F, Lacy E . Manipulating the Mouse Embryo-A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1986:250–76.
Fujiwara T, Nakada K, Shirakawa H, Miyazaki S . Development of inositol trisphosphate-induced calcium release mechanism during maturation of hamster oocytes. Dev Biol 1993; 156:69–79.
Swann K . A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertiliza- tion in hamster eggs. Development 1990; 110:1295–1302.
Mehlmann LM, Kline D . Regulation of intracellular calcium in the mouse egg: calcium release in response to sperm or inositol trisphosphate is enhanced after meiotic maturation. Biol Reprod 1994; 51:1088–9.
Carroll J, Swann K, Whittingham DG, Whitaker M . Spatiotemporal dynamics of intracellular [Ca2+]i oscillations during the growth and meiotic maturation of mouse oocytes. Development 1995; 120:3507–17.
Eppig JJ, Schultz RM, O'Brien M, Chesnel F . Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol 1994; 164:1–9.
Zernicka-Goetz M, Ciemerych MA, Kubiak JZ, Tarkowski AK . Cytostatic factor inactivation is induced by a calcium-dependent mechanism present until the second cell cycle in fertilized but not in parthenogenetically activated mouse eggs. J Cell Science 1995; 108:469–74.
Maleszewski M . Behavior of sperm nuclei incorporation into parthenogenetic mouse eggs prior to the first cleavage division. Molecular Reproduction and Development 1992; 33:215–21.
Jones K, Carroll J, Whittingham DG . Ionomycin, thapsigargin, ryanodine, and sperm induced Ca2+ release increase during meiotic maturation of mouse oocytes. J Biol Chem 1995; 270:6671–7.
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The work was supported by the Postdoctoral Science Foundation of China, the National Natural Science Foundation of China and in part by the Rockefeller Foundation of the United States.
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Deng, M., Sun, F. The fertilization-induced Ca2+ oscillation in mouse oocytes is cytoplasmic maturation dependent. Cell Res 6, 167–175 (1996). https://doi.org/10.1038/cr.1996.18
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DOI: https://doi.org/10.1038/cr.1996.18
