Figure 1 | Cell Research

Figure 1

From: SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis

Figure 1

SUVR2 is required for transgene and transposable element silencing. (A) The luminescence phenotype and kanamycin resistance of WT, ros1, ros1#87, and ros1nrpe1. The Arabidopsis seedlings were cold-treated at 4 °C for 2-4 days and subjected to luminescence imaging. Kanamycin sensitivity assay was carried out on MS medium supplemental with 150 mg/l kanamycin. (B) Semiquantitative RT-PCR was carried out to detect the RNA transcript levels of RD29A-LUC, endogenous RD29A, 35S-NPTII, and transposable elements in WT, ros1, ros1nrpe1, and ros1#87. The actin gene ACT7 was amplified as an internal control. No RT shows amplification of ACT7 using RNA samples as templates without reverse transcription, indicating no DNA contamination in the RNA samples. (C) The RNA transcript levels of solo LTR and AtGP1 as determined by quantitative RT-PCR.

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