Figure 3
Superposition of FabZ monomer subunits. In the structure of a FabZ-ACP dimer subunit, only one monomer is associated with ACP, termed monomer B; the other is termed monomer A. In the structure of the ACP-unbound FabZ hexamer (PDB code: 2GLL), three monomers adopt open conformation (monomers D, E and F), and the other three adopt close conformation (monomers A, B and C). (A) Superposition of FabZ monomer B (cyan) from FabZ-ACP complex with monomer E (yellow) from native FabZ hexamer structure (PDB code: 2GLL). The β-sheet layers from monomer B and E are colored in blue and orange, respectively. holo-ACP is colored in magenta, and the 4′-Pan-arm is shown in sticks. (B) Superposition of FabZ monomers A (green) and B (cyan) from the FabZ-ACP complex. The β-sheet layers from monomers A and B are colored in forest green and blue, respectively. (C-F) Superposition of FabZ β-sheet layers. Residues around the entrance are shown and labeled. (C) Superposition of monomer A (green) with monomer B (cyan) from FabZ-ACP complex. (D) Superposition of monomer A (yellow) from the native FabZ hexamer (PDB code: 2GLL) with monomer A (green) from the FabZ-ACP complex. (E) Superposition of monomer A (yellow) with monomer E (wheat), both from native FabZ hexamer structure. Atoms of Tyr100 and Pro112′ involved in the interactions are presented as transparent spheres. (F) Superposition of monomer E (wheat) from the native FabZ hexamer with monomer A (blue) from FabZ (Y100A) mutant hexamer (PDB code: 2GLV).
