Figure 4
RT-PCR analysis of AON-treated control cell cultures. AONs 19–2, 24–1, 24–2, 30–1, 30–2 and 34–1 are effective, whereas 19–1, 32, 34–2 and C (a control AON targeting the DMD (dystrophin) gene) are not. Correct exon skipping was confirmed using sequence analysis (data not shown). No exon 19, 24, 32 or 34 skipping could be observed in nontreated (NT) cells, whereas for exon 30 low levels of physiological skipping were observed. AON treatment significantly increased these levels from <10 to >90%. Skipping percentages using Lab-on-a-chip (Agilent, Amstelveen, the Netherlands) are indicated below each skip. Note that the intensity of the skip products is lower, due to the smaller fragment length (our efficiency assessment corrects for this). –RT and H2O are negative controls. M is size marker.
