Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Co-localization and interaction of human organic anion transporter 4 with caveolin-1 in primary cultured human placental trophoblasts

Figure 2

Morphology and characterization of primarily cultured placental trophoblasts. (A) Light microscopy of confluently grown placental trophoblasts. (B) Messenger RNA expression of cytokeratin-7, hOAT4 and caveolin-1 in the cultured placental trophoblasts. Total RNA (500 ng) isolated from cultured trophoblasts was reverse transcribed and PCR was performed (35 cycles). PCR products were separated using 1% agarose gel and stained with ethidium bromide. (C) Isolated membrane protein (30 µg/lane) was separated by electrophoresis and presence of each cytokeratin-7, hOAT4 and caveolin-1 was detected with respective antibodies. Data was shown typical result from 3 independent experiments. a; cytokeratin-7, b; hOAT4, c; caveolin-1.

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