Figure 5
The effects of Xenopus laevis caveolin-1 scrambled or antisense oligodeoxynucleotide on hOAT4-mediated [3H] estrone sulfate uptake in Xenopus laevis oocyte expression system. (A) The expression of caveolin-1 in scrambled or antisense ODN injected oocytes. Membrane protein was extracted from 50 ODN injected oocytes and Western blot analysis was performed. (B) Defolliculated stage VI and V oocyte were injected with water, with hOAT4 cRNA (30 ng/oocyte), with hOAT4+scrambled ODN (3 pmol/oocyte), or with hOAT4 antisense ODN (10 pmol/oocyte). After 3 days, [3H] estrone sulfate (100 nM) uptakes were determined for 1 h. Each bar represents the mean ± standard error for three experiments. Each experiment contains 8-10 oocytes.
