Figure 1
From: Down syndrome critical region 1 enhances the proteolytic cleavage of calcineurin
Increase of calcineurin cleavage by DSCR1. (A) Proteolysis of calcineurin by DSCR1 overexpression. SK-N-SH cells expressing endogenous calcineurin inhibitor proteins together with calcineurin were harvested and directly boiled in SDS sample buffer for western blot analysis. (B) Enhancement of endogenous calcineurin cleavage by DSCR1 overexpression. Mouse primary cortical neurons were infected with adenovirus expressing DSCR1 or empty adenovirus vector on the fourth day after plating. Three days later, cells were harvested and cell lysates were prepared by direct boiling in SDS sample buffer. (C) Enhancement of exogenous calcineurin cleavage by DSCR1. pSG-HA-calcineurin and pcDNA3-DSCR1-FLAG were transfected into HEK293 cells. After 24 h, cells were harvested and directly boiled in SDS sample buffer. (D) Increased proteolysis of calcineurin by DSCR1L1 (ZAKI-4) overexpression. Increasing concentrations of pSG-HA-DSCR1L1 with pSG-HA-calcineurin were transfected into HEK293 cells. After 24 h, cells were harvested and directly boiled in SDS sample buffer.
