Figure 7 | Experimental & Molecular Medicine

Figure 7

From: Ubiquitylation of Fe65 adaptor protein by neuronal precursor cell expressed developmentally down regulated 4-2 (Nedd4-2) via the WW domain interaction with Fe65

Figure 7

Protein stability of Fe65 (wt) and its WW domain mutant. EGFP-Fe65 or Fe65 WW domain mutant was transfected into HEK293 cells and the cells were treated with cyclohexamide. The Fe65 proteins were chased for the indicated time periods. Western blotting with a monoclonal Fe65 antibody of SDS-PAGE subjected EGFP-Fe65 proteins is shown in (A). To monitor the protein amount, an equal amount of cell lysate was subjected to western blotting with an actin antibody. Results shown are one of five repeated experiments. Quantification of the pulse-chase experiment is shown in (B) by image analysis with the Fuji Image Quant software.

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