Figure 4 | Experimental & Molecular Medicine

Figure 4

From: ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells

Figure 4

Tissue inhibitor of metalloproteinase-2 (TIMP-2) is a target of miR-19a and miR-96. (a) Hep3B cells were transfected with miR-19a mimics, miR-96 mimics, miR-19a inhibitors and miR-96 inhibitors. Expression of miR-19a, miR-96 and TIMP-2 was examined by quantitative real-time PCR (RT-qPCR) analysis. (b) After 24 h of transfection with miR-19a mimics, miR-96 mimics, miR-19a inhibitors and miR-96 inhibitors, cell extracts were prepared for western blotting. A representative blot from three independent experiments is shown. (c) Hep3B cells were transfected with miR-19a mimics, miR-96 mimics and adenine nucleotide translocase 2 (ANT2) short hairpin RNA (shRNA). Expression of TIMP-2 was examined using western blotting.

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