Figure 5 | Experimental & Molecular Medicine

Figure 5

From: ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells

Figure 5

miR-19a and miR-96 are regulated by the phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway in hepatocellular carcinoma (HCC). (a) After 24 h of transfection with scramble and adenine nucleotide translocase 2 (ANT2) short hairpin RNA (shRNA) in Hep3B cells, total RNA was extracted and subjected to quantitative real-time PCR (RT-qPCR). (b) Hep3B cells were transfected with scramble and ANT2 shRNA for 24 h, and western blotting was performed. β-actin was used as a loading control. (c) The levels of miR-19a and miR-96 were analyzed by RT-qPCR after treatment with LY294002, a PI3K inhibitor. (d) After treating Hep3B cells with LY294002, expression of tissue inhibitor of metalloproteinase-2 (TIMP-2) was examined by RT-qPCR analysis. Three independent experiments were performed, and the mean±s.d. is shown.

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