Figure 1

Downregulation of MMP1 mRNA expression by miR-526b. Human adult primary fibroblasts were transfected with 20-μM miR-526b mimic, the miR-1248 mimic or N.C. mimic. The expression of (a) miR-526b and (b) miR-1248 were analyzed using TaqMan miRNA RT-qPCR and normalized to the expression of rnu6b (U6). The expression of (c) MMP1, (d) MMP3, (e) MMP8 and (f) MMP13 mRNA were analyzed using RT-qPCR and normalized to the expression of GAPDH mRNA. The data are shown as the mean±s.e.m. of at least three independent experiments (*P<0.05 compared with N.C. treated cells). (g) Proteins of MMP-1 expressed in the serum-free conditioned media from fibroblasts transfected with miR-526b, miR-1248 or N.C. mimic were analyzed by western blot. (h) Fibroblasts transfected with miR-526b, miR-1248 or N.C. mimic were irradiated with UVB light at 60 mJ cm⁻². After 2 days, the cell viability was measured by WST-1 assay.