Figure 2

SERCA2 protein expression was regulated in a time-dependent manner in CCL21-treated DCs. (a) At the indicated time points (0, 15, 30 and 120 min) before DC collection, CCL21 (25 ng ml−1)-treated DCs were prepared and analyzed via western blotting using antibodies against SERCA2, PLB and Hax-1. An anti-tubulin antibody was used as the internal control. The data are representative of three independent experiments. (b) RT-PCR analysis was performed using DCs treated with CCL21 for 2 h before getting collected. The data are presented as the means±s.d. of three independent experiments.