Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) reduces the migratory capacity of CCL21-treated monocyte-derived dendritic cells

Figure 3

The levels of SERCA2 expression determined the migratory capacities of DCs. (a) Immature monocyte-derived DCs were transduced with adenovirus-mediated SERCA2 siRNA or scrambled siRNA and then matured using the cytokine cocktails described in the Materials and methods. mDCs were collected and analyzed via Western blot and migration assays. (b) Immature monocyte-derived DCs were transduced with SERCA2- or GFP-overexpressing adenoviruses and then matured using the cytokine cocktails described in the Materials and methods. (c) Cyclopiazonic acid was administered during DC maturation. Western blot results are representative of three independent experiments, and results of the migration assays are presented as the means±s.d. of three independent experiments. P-values indicate significant differences compared with the controls.

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