Figure 5 | Experimental & Molecular Medicine

Figure 5

From: Sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) reduces the migratory capacity of CCL21-treated monocyte-derived dendritic cells

Figure 5

CCL21 differentially regulated SERCA2 expression and cofilin phosphorylation in the presence of various maturation cytokines or TLR agonists. (a) At the indicated time points, CCL21-treated mDCs were collected and a western blot analysis was performed as described in the Materials and methods. (b) Immature DCs were stimulated with various cytokines or TLR agonists for 48 h, after which, mature DCs were collected for western blot and migration assays. (c) The immunoreactive bands were analyzed and quantitated using an LAS-3000 imaging system and Multi Gauge version 3.0, respectively. Western blot results are representative of three independent experiments, and migration data are presented as the means±s.d. of three independent experiments.

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