Table 1 Primer sequences of PCR

From: Effects of microRNA-135a on the epithelial–mesenchymal transition, migration and invasion of bladder cancer cells by targeting GSK3β through the Wnt/β-catenin signaling pathway

Genes

Sequences (5′→3′)

miR-135a

F:AGGCCTCGCTGTTCTCTATGGC

 

R:TGTCCCCGCCGTGCG

GSK3β

F:GGCAGCATGAAAGTTAGCAGA

 

R:GGCGACCAGTTCTCCTGAATC

β-Catenin

F:TGGTGACAGGGAAGACATCA

 

R:CCATAGTGAAGGCGAACTGC

CyclinD1

F:TCAAGTGTGACCCGGACTGCCT

 

R:GCACGTCGGTGGGTGTGCAA

E-cadherin

F:AACGCATTGCCACATACAC

 

R:GAGCACCTTCCATGACAGAC

Vimentin

F:ACAGGCTTTAGCGAGTTATT

 

R:GGGCTCCTAGCGGTTTAG

GAPDH

F:GGTGAAGGTCGGAGTCAACGG

 

R:CCTGGAAGATGGTGATGGGATT

U6

F:CTCGCTTCGGCAGCACA

 

R:AACGCTTCACGAATTTGCGT

  1. Abbreviations: F, forward; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; R, reverse.
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