Fig. 3
From: A feasibility study for the newborn screening of spinal muscular atrophy
A representative real-time reaction showing the real-time multiplex polymerase chain reaction analysis of three blood spots including an affected individual, a spinal muscular atrophy carrier, and normal individuals at RNase P (A) and SMN1 (B). All SMN1 and SMN2 copy numbers were independently verified using a competitive polymerase chain reaction (data not shown, see “Materials and Methods”). Gene copy numbers, corresponding amplification curve colors, and CT values for each sample (table at right).
