Fig. 4
From: A feasibility study for the newborn screening of spinal muscular atrophy
Real-time multiplex amplification of a series of DNA extractions from blood spots including a normal sample and a series of affected individuals with SMN2 copies ranging from one to five. Again, SMN1 and SMN2 copy numbers were determined by using independent polymerase chain reaction methods (data not shown, see “Material and Methods”). Gene copy numbers, corresponding amplification curve colors, and CT values for RNase P (A) and SMN1 (B) for each sample (table at right).
