Summary
Polyacrylamide gel isoelectric focusing was used to study the buffer-soluble proteins of stigmatic homogenates of six S-allele genotypes in Brassica oleracea L. More than nine protein bands at higher pH in gels, being distinctly separated, were taken for the analysis of S-allele specificity. S-allele specificity was ascribable to a combination of the protein bands, and the parental bands were transmitted to the hybrid F1s. These protein bands appeared in the course of stigma maturation. No specific band pattern was observed in the leaf and seedling proteins, however. It is postulated that the S-allele produces several fractions of proteins and S-allele specificity is expressed by a combination of the protein fractions.
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Nishio, T., Hinata, K. Analysis of S-specific proteins in stigma of Brassica oleracea L. by isoelectric focusing. Heredity 38, 391–396 (1977). https://doi.org/10.1038/hdy.1977.102
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DOI: https://doi.org/10.1038/hdy.1977.102
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