Abstract
Variation in high molecular weight (HMW) glutenin subunit composition among Aegilops species of the Sitopsis section, namely Ae. bicornis, Ae. longissima, Ae. sharonensis, Ae. searsii and Ae. speltoides was investigated using one-dimensional, as well as two step one-dimensional (2S-1D), sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The presence of three glutenin bands in several accessions of Ae longissima and Ae. sharonensis seems to be evidence for the existence of at least three active genes, called x, y and z, controlling the synthesis of the high molecular weight glutenins in the genome S of Aegilops. From comparisions between the glutenin patterns found in the species analysed and those bands observed for the B genome of hexaploid wheat, it can be concluded that Ae. bicornis9 Ae. longissima and Ae. sharonensis are unlikely donors of the B genome, mainly because of the presence of specific glutenin markers that were absent in wheat. Ae. searsii and Ae. speltoides showed glutenin subunits that moved within the range of those of B, but Ae. speltoides is the only species that could explain the variablity for HMW subunits previously described for the B genome of wheat, and therefore it cannot be excluded as a possible donor of this genome.
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Fernández-Calvín, B., Orellana, J. High molecular weight glutenin subunit variation in the Sitopsis section of Aegilops. Implications for the origin of the B genome of wheat. Heredity 65, 455–463 (1990). https://doi.org/10.1038/hdy.1990.117
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DOI: https://doi.org/10.1038/hdy.1990.117
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