Figure 4
Structure of SPE31 (a) and alignment of P34, SPE31 and several significant ancestral nodes (b). The same color is assigned to each same residue. The color red is assigned to the conserved catalytic triad residues (Gly26 is also colored red); blue to Phe169 of SPE31, which disrupts catalytic activity; purple to the N-glycosylation site (Asn159 of SPE31) binding the three glycosyl residues (purple also); and yellow signifies the four sites that bind a natural peptide (yellow also) in SPE31. In addition, a was prepared using PyMOL (http://www.pymol.org/). In b , the sites in the ancestral nodes with maximum probabilities of <0.9 are indicated by dot. Green represents all of the different sites ranging from nodes I to VI. The asterisk indicates the positive selection sites identified by branch-site models.
