Table 1 Descriptive statistics of COI and 16 microsatellite loci for each collection site of Cumberlandia monodonta from populations in the Clinch River, TN; Gasconade and Meramec Rivers, MO; Ouachita River, AR; St. Croix River, MN; Tennessee River, AL; and a single specimen in the Cumberland River, KY

From: Phylogeographic and population genetic analyses reveal Pleistocene isolation followed by high gene flow in a wide ranging, but endangered, freshwater mussel

Population

 

COI

Microsatellite

 

n

H

H R

H P

K

Ï€

%Lineage 1

N A

A R

A P

H O

H E

θ

N e

Clinch

48

18

11.0 (1.45)

11

4.17

0.0062

71

13.9 (1.77)

12.6 (1.49)

22

0.770 (0.034)

0.850 (0.028)

8.750 (8.146–9.400)

4375 (4073–4700)

Gasconade

40

9

6.6 (1.05)

6

3.18

0.0047

82

12.8 (1.62)

11.8 (1.40)

8

0.829 (0.024)

0.841 (0.024)

8.811 (8.185–9.501)

4406 (4093–4751)

Meramec

34

8

7.2 (0.98)

3

2.97

0.0044

83

13.8 (1.78)

12.7 (1.50)

19

0.815 (0.028)

0.854 (0.022)

11.094 (10.245–12.046)

5547 (5123–6023)

Ouachita

20

3

3.0 (0.00)

3

0.48

0.0007

0

8.0 (1.15)

7.9 (1.12)

12

0.702 (0.061)

0.737 (0.041)

2.820 (2.648–3.006)

1410 (1324–1503)

St. Croix

50

9

6.5 (1.07)

4

2.25

0.0033

90

13.2 (1.69)

12.1 (1.45)

8

0.804 (0.032)

0.842 (0.026)

9.118 (8.438–9.868)

4559 (4219–4934)

Tennessee

5

4

—

0

2.00

0.0030

100

—

 

—

—

—

—

—

Cumberland

1

1

—

0

—

—

100

—

 

—

—

—

—

—

Total

198

32

9.6 (1.61)

—

3.83

0.0059

74

12.3 (0.75)

12.8 (1.55)

69

0.784 (0.017)

0.825 (0.014)

—

—

  1. Abbreviations: AP, number of private alleles; AR, rarefied allelic richness; COI, cytochrome oxidase subunit I; H, number of haplotypes; HE, mean expected heterozygosity; HO, mean observed heterozygosity; HP, number of private haplotypes; HR, rarefied number of haplotypes; K, mean number of base pair differences; n, number of samples; NA, mean number of observed alleles; π, nucleotide diversity; %Lineage 1, percentage of Lineage 1 haplotypes; θ, effective population size (Ne) parameter (θ=4Neμ, where μ is mutation rate per generation). Ne estimates were based on a mutation rate of 5 × 10−4 per generation. (s.d.), except θ and Ne have 95% confidence interval.