Figure 1

Standardized cyto-nuclear LD (D′) between 14 nuclear loci and a SNP in the mitochondrial gene atp1 found in populations of S. latifolia sampled in 1993 and 2008 with a well-characterized metapopulation located in Giles County, VA, USA. Values of D′ can range from –1.0 to 1.0. Within the sampled plant populations, D′ was found to range from –0.424 to 0.6211 in 1993 and –0.6316 to 0.3888 in 2008. There was a general trend for an overall decrease in D′ between sampling periods, though only slat85 showed a significant (positive) change in overall cyto-nuclear D′ based upon our t-test of samples (*P<0.05 after Benjamini–Hochberg correction). slat33, slat72 and SL_eSSR20 all showed a significant decrease in D′.