Table 1 Summary of the COI (upper) and ITS-1 (lower) nucleotide diversity estimates and neutrality tests

Locality	n	S	h	π	Tajima’s D	Fu’s F_S	R ₂	Z _nS
COI
01-AR	11	107	8	0.0650	1.464	4.797	0.245**	0.577
04-ED	36	12	7	0.0050	1.346	2.558	0.165	0.358
07-SO	44	20	15	0.0045	−0.758	−3.28	0.087	0.067
10-SB	10	31	6	0.0109	−0.993	1.842	0.205	0.481
13-EC	13	4	4	0.0019	0.792	0.514	0.191	0.425
16-BB	25	6	7	0.0011	−1.159	−3.176*	0.082	0.013
19-PC	20	81	13	0.0461	2.167*	3.964	0.228**	0.711**
22-PI	22	6	6	0.0016	−0.686	−1.367	0.103	0.166
25-SL	9	99	7	0.0530	0.549	3.536	0.27	0.476
28-SI	15	61	10	0.0115	−2.283**	−0.157	0.232**	0.885**
31-ST	8	67	5	0.0457	2.464**	6.335*	0.279**	0.913**
All localities	213	226	88	0.0754	—	—	—	—
ITS-1
01-AR	8	67/72	4	0.0436	−1.829**	6.094*	0.315**	0.927**
04-ED	21	8/26	8	0.0029	−1.587	−4.204**	0.068**	0.120
07-SO	10	7/21	6	0.0048	−1.116	−2.082	0.112*	0.188
10-SB	9	0/1	1	0	—	—	—	—
13-EC	10	3/3	2	0.0014	−1.562	1.225	0.3	1**
16-BB	22	1/3	2	0.0002	−1.162	−0.957	0.208*	—
19-PC	11	59/64	5	0.0682	1.826*	9.334**	0.243*	0.850**
22-PI	12	12/21	6	0.0058	−1.865*	−0.849	0.156	0.306
25-SL	2	0/0	1	0	—	—	—	—
28-SI	11	35/40	2	0.0150	−2.181**	9.321**	0.287**	1**
31-ST	5	61/63	4	0.0841	1.567	3.843	0.283	0.945**
All localities	121	86/176	28	0.0673	—	—	—

Abbreviations: COI, cytochrome oxidase I; ITS-1, internal transcribed spacer-1.
For ITS-1, the first and second values of S indicate the number of polymorphic sites under the complete deletion or using all positions, respectively.
*P<0.05, **P<0.01 (two-tailed test).

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