Figure 5

(a) A sequence gap between Bm_scaf43 and Bm_scaf 103 of B. mori chromosome 11 revealed by BAC-FISH. Signals from 17J12F (green), 19D05C (magenta) 04L10D (yellow) and 04A07H (red) represent AK386872, AK385865, NM_001043588.1 and BP125410, respectively. Note that the green signals from 17J12F faintly stain the heterochromatic region (refer to Figure 2 in Yoshido et al. 2005) of B. mori chromosome 11 (green arrows). (b) Ectopic signals from an O. furnacalis fosmid (76F08) located near the end of chromosome 30 appear in O. nubilalis chromosome 30 (Chr30 shown in white letters). Two fosmids, 76F08 and 14J14, carrying representative single genes, LC002996 and LC003001, are orthologues of B. mori AK383286 and NM_001043790.1, respectively (see Table 1). Black bars represent B. mori chromosomes 11 and 23 (Chr11 and Chr23). Scale bars, 5 μm.