Figure 3 | Horticulture Research

Figure 3

From: Assessing the allelotypic effect of two aminocyclopropane carboxylic acid synthase-encoding genes MdACS1 and MdACS3a on fruit ethylene production and softening in Malus

Figure 3

Agarose gel analyses of markers ACS1 (a), CAPS866 (b) and CAPS870 (c). For marker ACS1, the PCR products amplified by primers ACS1–5F/R were directly analyzed. Allelotypes MdACS1-1/1, MdACS1–2/2 and MdACS1-1/2 are denoted with ‘1/1’, ‘2/2’ and ‘1/2’, respectively. For marker CAPS866, the PCR products were first amplified by primers ACS3a-289F/R and then digested with enzyme BstNI, which restricts the MdACS3a (G866) allele into the two lower bands. Allelotypes MdACS3a/MdACS3a (G866/G866), MdACS3a/MdACS3a-G289V (G866/T866) and MdACS3a-G289V/G289V (T866/T866) are noted with ‘G/G’, ‘G/T’ and ‘T/T’, respectively. For marker CAPS870, enzyme TaqαI restricts the Mdacs3a (T870) allele into the two lower bands. Allelotypes MdACS3a/MdACS3a (C870/C870), MdACS3a/mdacs3a (C870/T870) and mdacs3a/mdacs3a (T870/T870) are noted with ‘C/C’, ‘C/T’ and ‘T/T’, respectively.

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