Figure 1
Effects of human coupling factor 6 (CF6) on the gene and protein expression of CXC chemokine receptor type 4 (CXCR4) in human umbilical vein endothelial cells (HUVECs). (a) Chronological changes after exposure to CF6 at 10−7 M (n=6). P<0.05 vs. 0 h. (b) Dose-related changes after a 24-h exposure to CF6 at 10−9–10−7 M (n=6). P<0.05 vs. CF6 (−). (c) Effects of CF6 at 10−7 M on the ratio of CXCR4 to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA at 24 h and the effect of various compounds on the CF6-induced decrease in CXCR4 gene expression (n=4–12). P<0.05 vs. control (without agonists). PP1 at 50 μM; αAb: antibody for α-subunit of ATP synthase (dilution of 1:1000); βAb: antibody for β-subunit of ATP synthase (dilution of 1:1000); small interfering RNA (siRNA): after a 40-h treatment with annealing siRNA targeting hypoxia-inducible factor (HIF)-1α at 100 nM. (d) Effect of CF6 and/or hypoxia on the ratio of CXCR4 to GAPDH mRNA at 24 h (n=6). P<0.05 vs. normoxia without CF6. (e) Representative bands for CXCR4 protein and the ratio of CXCR4 to GAPDH in the presence and absence of CF6 at 10−7 M for 24 h (n=4). P<0.05 vs. CF6 (−).
