Figure 2 | Hypertension Research

Figure 2

From: Coupling factor 6 attenuates CXCR4 expression through the HIF-1α and c-Src pathways and promotes endothelial apoptosis and inflammation

Figure 2

Mechanism of coupling factor 6 (CF6)-induced decrease in CXC chemokine receptor type 4 (CXCR4) in human umbilical vein endothelial cells (HUVECs). (a) Chromatin immunoprecipitation (ChIP) assay performed with primers encompassing the hypoxia response element (HRE) in the presence or absence of CF6 at 10−7 M for 7 h. An anti-hypoxia-inducible factor (HIF)-1α antibody (dilution of 1:40) was used to immunoprecipitate the associated chromatin. (b) Histone deacetylase 3 (HDAC3) in immunoprecipitates using an anti-phospho-c-Src (pcSrc) antibody after a 30-min exposure of HUVECs to CF6 at 10−7 M (n=4). PcSrc after a 30-min exposure to CF6 at 10−7 M (n=4). HDAC3 and PcSrc were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in the sample before immunoprecipitation. P<0.05 vs. CF6 (−). (c) ChIP assay performed with primers encompassing the HRE in the presence or absence of CF6 at 10−7 M for 7 h. An anti-HDAC3 antibody (dilution of 1:40) was used to immunoprecipitate the associated chromatin.

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