Figure 6
Reducing agent (N-acetylcysteine (NAC)) and BH4 reverse eNOS uncoupling SHR-HI. (a–c) Total aortic ROS production measured by electron paramagnetic resonance (EPR) in freshly frozen aorta homogenates pretreated with NAC (2 mM) or left untreated. (d) Total aortic ROS production measured by EPR in freshly frozen aorta homogenates pretreated with BH4 (100 μM) or left untreated. (e) Dose-dependent response to the cumulative addition of ACh on aortic rings pretreated with indomethacin+TRAM 34+apamin and BH4. The data are expressed as the percentage of relaxation relative to the preconstriction level. (f) Half-maximal effective concentration of ACh on aortic rings pretreated with ITA and BH4. (g) Maximal relaxation to ACh in aortic rings pretreated with ITA and BH4. The values are the mean±s.e.m. *P<0.05 vs. WKY group. †P<0.05 vs. SHR group. ‡P<0.05 vs. SHR-HI group. §P<0.05 vs. SHR treated with NAC or BH4.
