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Characterization and predicted role of the microRNA expression profile in amnion from obese pregnant women

International Journal of Obesity volume 38, pages 466–469 (2014)Cite this article

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Abstract

Maternal obesity and nutrient excess in utero increase the risk of future metabolic diseases. The mechanisms underlying this process are poorly understood, but probably include genetic, epigenetic alterations and changes in fetal nutrient supply. We have studied the microRNA (miRNA) expression profile in amnion from obese and control women at delivery to investigate if a specific miRNA signature is associated with obesity. The expression profile of 365 human miRNAs was evaluated with the TaqMan Array in amnion from 10 obese and 5 control (prepregnancy body mass index (BMI) >30 and <25 kg m−2, respectively) women at delivery. Target genes and miRNA-regulated pathways were predicted by bioinformatics. Anthropometric and biochemical parameters were also measured in mothers and newborns. Seven miRNAs were expressed only in obese women (miR-422b, miR-219, miR-575, miR-523, miR-579, miR-618 and miR-659), whereas 13 miRNAs were expressed at a higher level and 12 miRNAs at a lower level in obese women than in controls. MicroRNAs significantly downregulated the neurotrophin, cancer/ErbB, mammalian target of rapamycin, insulin, adipocytokine, actin cytoskeleton and mitogen-activated protein kinase signaling pathways. In conclusion, we show that the miRNA profile is altered in amnion during obesity and hypothesize that this could affect pathways important for placental growth and function, thereby contributing to an increase in the newborn’s risk of future metabolic diseases.

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Acknowledgements

This work was supported by grants from CEINGE Regione Campania (DGRC 1901/2009), IRCCS Fondazione SDN, Ministry of Health and POR Campania FSE 2007–2013 and Project CREME. We thank Jean Ann Gilder (Scientific Communication srl, Naples, Italy) for revising and editing the manuscript.

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Author notes

  1. C Nardelli and L Iaffaldano: These authors contributed equally to this work.

Authors and Affiliations

  1. Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Naples, Italy

    C Nardelli, L Iaffaldano, V Capobianco, R Di Noto, L Del Vecchio & L Pastore

  2. CEINGE-Biotecnologie Avanzate SCa RL, Naples, Italy

    C Nardelli, L Iaffaldano, M Ferrigno, V Capobianco, R Di Noto, L Del Vecchio & L Pastore

  3. IRCCS Fondazione SDN—Istituto di Ricerca Diagnostica e Nucleare, Naples, Italy

    G Labruna

  4. Dipartimento di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Naples, Italy

    G M Maruotti, F Quaglia & P Martinelli

  5. Laboratori misti, IRCCS Fondazione SDN—CEINGE, CEINGE—Biotecnologie Avanzate, Naples, Italy

    L Sacchetti

Authors
  1. C Nardelli
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  2. L Iaffaldano
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  3. M Ferrigno
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  4. G Labruna
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  5. G M Maruotti
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  6. F Quaglia
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  7. V Capobianco
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  8. R Di Noto
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  9. L Del Vecchio
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  10. P Martinelli
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  11. L Pastore
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  12. L Sacchetti
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Corresponding author

Correspondence to L Sacchetti.

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Nardelli, C., Iaffaldano, L., Ferrigno, M. et al. Characterization and predicted role of the microRNA expression profile in amnion from obese pregnant women. Int J Obes 38, 466–469 (2014). https://doi.org/10.1038/ijo.2013.121

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  • DOI: https://doi.org/10.1038/ijo.2013.121

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