Figure 1
From: KDM6B epigenetically regulates odontogenic differentiation of dental mesenchymal stem cells
KDM6B promotes odontogenic potential of dental MSCs. (a) Knockdown of KDM6B by shRNA. MSC/Scrsh, MSCs expressing scramble shRNA; MSC/KDM6Bsh2, MSCs expressing KDM6B shRNA2; MSC/KDM6Bsh3, MSCs expressing KDM6B shRNA3. (b) Knockdown of KDM6B inhibited ALP activities in MSCs. (c) Knockdown of KDM6B inhibited mineralization in MSCs. (d) Knockdown of KDM6B inhibited OSX expression in MSCs as determined by real-time RT-PCR. (e) Knockdown of KDM6B inhibited ALP expression in MSCs. (f) Knockdown of KDM6B inhibited OCN expression in MSCs. (g) Knockdown of KDM6B inhibited OPN expression in MSCs. ALP, alkaline phosphate; MSC, mesenchymal stem cell; RT-PCR, reverse transcription-polymerase chain reaction.
