Abstract
Isotope-labelling experiments have changed the way microbial ecologists investigate the ecophysiology of microbial populations and cells in the environment. Insight into the ‘uncultivated majority’ accompanies methodology that involves the incorporation of stable isotopes or radioisotopes into sub-populations of environmental samples. Subsequent analysis of labelled biomarkers of sub-populations with stable-isotope probing (DNA-SIP, RNA-SIP, phospholipid-derived fatty acid-SIP) or individual cells with a combination of fluorescence in situ hybridization and microautoradiography reveals linked phylogenetic and functional information about the organisms that assimilated these compounds. Here, we review some of the most recent literature, with an emphasis on methodological improvements to the sensitivity and utility of these methods. We also highlight related isotope techniques that are in continued development and hold promise to transform the way we link phylogeny and function in complex microbial communities.
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Acknowledgements
This work was supported by funding from the Natural Environment Research Council (United Kingdom). JDN also acknowledges support from the Natural Sciences and Engineering Research Council (Canada). MW was supported by the Vienna Science and Technology Fund (WWTF) and the Austrian Science Fund (FWF).
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Neufeld, J., Wagner, M. & Murrell, J. Who eats what, where and when? Isotope-labelling experiments are coming of age. ISME J 1, 103–110 (2007). https://doi.org/10.1038/ismej.2007.30
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DOI: https://doi.org/10.1038/ismej.2007.30
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