Figure 3
Microbial diversity in biofilm and suspended community, as estimated by T-RFLP (a) and 454 pyrosequencing (b), calculated as richness and the number equivalents of the Shannon entropy. A threshold of 0.2% contribution to the community was applied to the 454 pyrosequencing data to compare results from 454 pyrosequencing with T-RFLP analysis (c). con, confluence stream; hum, humic stream; out, outflow stream. Cross-hatched bars represent the biofilm community, solid bars the suspended community, green bars the bulk community and blue bars the active community.
