Abstract
A pair of genes encoding the bacterial two-component regulatory system, orf22 and orf23, was found next to the clavulanic acid gene cluster of Streptomyces clavuligerus NRRL3585. Orf23 was deleted for the construction of S. clavuligerus/Δorf23. Although growth and morphological analyses showed no differences between S. clavuligerus/Δorf23 and wild-type, the production of clavulanic acid in S. clavuligerus/Δorf23 was found to be decreased. In addition, the co-overexpression of orf22/orf23 in wild-type resulted in an enhanced 1.49-fold production of clavulanic acid, and the complementation of orf22/orf23 in S. clavuligerus/Δorf23 restored clavulanic acid production about 80% as normal levels. These results demonstrate that the orf22/orf23 two-component regulatory system participates as a positive regulator of the biosynthesis of clavulanic acid and increased levels of orf22/orf23 can contribute to enhanced production of clavulanic acid in S. clavuligerus.
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Jnawali, H., Oh, TJ., Liou, K. et al. A Two-component Regulatory System Involved in Clavulanic Acid Production. J Antibiot 61, 651–659 (2008). https://doi.org/10.1038/ja.2008.92
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DOI: https://doi.org/10.1038/ja.2008.92
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