Abstract
CXCL10 (inducible protein-10) is a highly inducible chemoattractant, which contributes to the recruitment of inflammatory cells, such as macrophages and T-lymphocytes, and thereby has important roles in chronic inflammatory conditions. In a search for new inhibitors of CXCL10 expression in MonoMac6 cells, a novel compound, designated as Ganodermycin, was isolated from fermentations of the basidiomycete Ganoderma applanatum. The structure was determined by a combination of spectroscopic techniques. Ganodermycin inhibited the lipopolysaccharide (LPS)/interferon (IFN)-γ-induced CXCL10 promoter activity in transiently transfected MonoMac6 cells in a dose-dependent manner with IC50 values of 15–20 μg ml−1 (53–71 μM). Ganodermycin also reduced LPS/IFN-γ-induced CXCL10 protein synthesis and excretion.
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Acknowledgements
This work was supported by a grant from the Stiftung Rheinland-Pfalz für Innovation. We thank Professor Dr T Anke for providing Gonoderma applanatum (IBWF09003), Dr N Hanold for mass spectrometric analyses and A Meffert for expert technical assistance.
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Jung, M., Liermann, J., Opatz, T. et al. Ganodermycin, a novel inhibitor of CXCL10 expression from Ganoderma applanatum. J Antibiot 64, 683–686 (2011). https://doi.org/10.1038/ja.2011.64
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DOI: https://doi.org/10.1038/ja.2011.64
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