Abstract
Two new butenolides, designated trichocyalides A and B, were isolated along with the known compound harzianolide, from the culture broth of Trichoderma sp. FKI-5513 by solvent extraction, ODS column chromatography and HPLC. Their structures were elucidated by several spectral analyses, showing that they have the common skeleton of butenofuranone. Trichocyalides A and B inhibited alkaline phosphatase (ALP) activity, a typical marker enzyme of osteoblastic differentiation (IC50: 83.0 and 187 μM, respectively), in bone morphogenetic protein (BMP)-stimulated C2C12 myoblasts mutant cells, which stably express BMP receptor activity, whereas harzianolide showed no inhibitory activity against ALP even at 500 μM.
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We wish to thank Dr K Nagai and Ms N Sato (Kitasato University) for measurements of mass and NMR spectra, respectively.
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Fukuda, T., Uchida, R., Ohte, S. et al. Trichocyalides A and B, new inhibitors of alkaline phosphatase activity in bone morphogenetic protein-stimulated myoblasts, produced by Trichoderma sp. FKI-5513. J Antibiot 65, 565–569 (2012). https://doi.org/10.1038/ja.2012.70
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DOI: https://doi.org/10.1038/ja.2012.70
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