Figure 1
From: MA026, an anti-hepatitis C virus compound, opens tight junctions of the epithelial cell membrane
MA026 irreversibly increased TJ permeability in the MDCKII cell monolayer. (a) the structure of MA026. (b) cytotoxicity assay against MDCKII monolayer. MDCKII cells were cultured for 3 days to establish monolayer integrity, and treated with several concentrations of MA026 for 4 h. (c) MA026 irreversibly increased paracellular permeability of FD4 in the MDCKII monolayer. The final concentrations were 0 μM (Δ), 1 μM (
), and 3 μM (
) of MA026, or 0.1 μM LatA (○). The representative data of two independent experiments are shown. (d) MA026 decreased the TER of MDCKII monolayer. The final concentrations were 0 μM (Δ) and 3 μM (
) of MA026, or 0.1 μM LatA (○). The representative data of 3 independent experiments are shown.
